Expression detection of DMRTs and two sox9 genes in Takifugu rubripes (Tetraodontidae, Vertebrata)

Autor:	Jian-Zhou Cui, Xue-Yan Shen, Qingli Gong, Guanpin Yang, Qianqun Gu
Rok vydání:	2007
Předmět:	Genetics Sexual differentiation biology Takifugu rubripes Ocean Engineering Ovary SOX9 Oceanography biology.organism_classification Molecular biology Real-time polymerase chain reaction medicine.anatomical_structure Gene expression medicine Tetraodontidae Gene
Zdroj:	Journal of Ocean University of China. 6:182-186
ISSN:	1993-5021 1672-5182
DOI:	10.1007/s11802-007-0182-7
Popis:	Sex determination and sex differentiation are important phenomena in fish, but the mechanisms of sex determination in Takifugu rubripes are poorly understood. In our study, the expression patterns of genes for DMRTs (DMRT1, DMRT2 and DMRT3), sox9a and sox9b in T. rubripes tissues were verified with the Reverse Transcription (RT)-PCR detection. It is showed that DMRT1 expressions in testis and ovaries were much lower, and no expressions were found in muscle, blood and tailfin. However, expressions for DMRT2 and DMRT3 were not found in the tissues stated above. Transcripts of sox9a were detected in muscle, fin, ovary and testis, but not in blood, whereas sox9b expression was only detected in ovary. The expression patterns of DMRTs, sox9a and sox9b in T. rubripes gonads suggest that these genes may not be sex-specific.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::88874ee4731f90cd6d898f0a92918584 https://doi.org/10.1007/s11802-007-0182-7 Zobrazit plný text záznamu Full text from SpringerLink