In vivo continuous three-dimensional magnetic resonance microscopy: a study of metamorphosis in Carniolan worker honey bees (Apis mellifera carnica)
| Autor: | Polona Mrak, Janko Božič, Urša Mikac, Igor Serša, Chenyun Lin, Ana Sepe, Kaja Tušar, Aleš Mohorič, Georgy Mikhaylov |
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| Rok vydání: | 2020 |
| Předmět: |
0303 health sciences
Larva Physiology Magnetic resonance microscopy media_common.quotation_subject Honey bee Aquatic Science Biology 030218 nuclear medicine & medical imaging Gray level 03 medical and health sciences Honey Bees 0302 clinical medicine In vivo Insect Science Time course Animal Science and Zoology Metamorphosis Biological system Molecular Biology Ecology Evolution Behavior and Systematics 030304 developmental biology media_common |
| Zdroj: | Journal of Experimental Biology. |
| ISSN: | 1477-9145 0022-0949 |
| DOI: | 10.1242/jeb.225250 |
| Popis: | Three-dimensional (3D) magnetic resonance microscopy (MRM) is a modality of magnetic resonance imaging (MRI) optimized for the best resolution. Metamorphosis of the Carniolan worker honey bee (Apis mellifera carnica) was studied in vivo under controlled temperature and humidity conditions from sealed larva until the emergence of an adult. The 3D images were analyzed by volume rendering and segmentation, enabling the analysis of body, tracheal system and gastrointestinal tract through volume time course. Fat content sensitivity enabled the analysis of flight muscles transformation during the metamorphosis by the signal histogram and gray level co-occurrence matrix (GLCM). Although the transformation during metamorphosis is well known, MRM enables an alternative insight to this process, i.e., 3D in vivo which has relatively high spatial and temporal resolutions. The developed methodology can easily be adapted for studying metamorphosis of other insects or any other incremental biological process on a similar spatial and temporal scale. |
| Databáze: | OpenAIRE |
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