Applying Mesoporous Silica SBA-15 in Electrochemical Detection of DNA Hybridization
| Autor: | Miroslav Fojta, Bohdan Josypčuk, Oksana Josypčuk, Aleš Daňhel |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Materials science
DNA–DNA hybridization 010401 analytical chemistry Nanotechnology 02 engineering and technology Electrochemical detection Mesoporous silica 021001 nanoscience & nanotechnology 01 natural sciences 0104 chemical sciences Analytical Chemistry Chemical engineering Covalent bond Cathodic stripping voltammetry Electrochemistry 0210 nano-technology Voltammetry |
| Zdroj: | Electroanalysis. 28:1860-1864 |
| ISSN: | 1040-0397 |
| DOI: | 10.1002/elan.201501137 |
| Popis: | Mesoporous silica SBA-15 powder was used as the separation surface for hybridization purposes and it has proved appropriate and effective. By help of a simple procedure any oligodeoxynucleotide (ODN) modified by NH2-group can be covalently bound at the surface of SiO2 particles, what significantly extends the capabilities of the experimenter. Use of cathodic stripping voltammetry for electrochemical detection of ODN/DNA hybridization ensures high sensitivity of the analyses. Only ordinary laboratory equipment is needed for the implementation of these experiments. Due to the very small consumption of SBA-15, commonly sold quantity (5 g) may be sufficient for many years of work. |
| Databáze: | OpenAIRE |
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