In vitro production of high titre monoclonal antibody by hybridoma cells in dialysis culture

Autor:	S. R. Adamson, G. M. Gaucher, Leo A. Behie, B. H. Lesser, S. L. Fitzpatrick
Rok vydání:	1983
Předmět:	Growth medium Fetus Programmed cell death Cell growth medicine.drug_class Bioengineering General Medicine Biology Monoclonal antibody Applied Microbiology and Biotechnology Molecular biology In vitro chemistry.chemical_compound Titer chemistry Cell culture medicine Biotechnology
Zdroj:	Biotechnology Letters. 5:573-578
ISSN:	1573-6776 0141-5492
DOI:	10.1007/bf00130835
Popis:	Relatively high levels of nutrients and low levels of waste products were maintained in cultures of hybridoma cells by dialysis against growth medium supplemented with fetal calf serum (FCS). This resulted in a greater than ten fold increase in cell density and monoclonal antibody titre compared to conventional batch cultures. Exclusion of FCS from the dialysing growth medium resulted in cell death, indicating that a low molecular weight serum component was necessary for growth. This study indicates that nutrient depletion limits hybridoma cell growth in conventional batch culture and also provides a simple and economical means of producing relatively high titre monoclonal antibody in vitro.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::882bb32c6c05e3a22a84f83765c956af https://doi.org/10.1007/bf00130835 Zobrazit plný text záznamu Full text from SpringerLink