Optimization and characterization of toroidal foci for super-resolution fluorescence microscopy: tutorial

Autor:	Lucía F. Lopez, Luciano A. Masullo, Alan M. Szalai, Florencia Edorna, Florencia D. Choque, Fernando Caprile, Fernando D. Stefani
Rok vydání:	2023
Předmět:	Statistical and Nonlinear Physics Atomic and Molecular Physics and Optics
Zdroj:	Journal of the Optical Society of America B. 40:C103
ISSN:	1520-8540 0740-3224
DOI:	10.1364/josab.482413
Popis:	Single-molecule localization microscopy (SMLM) has become an essential tool to investigate phenomena at the nanoscale. Among the different SMLM approaches, methods that interrogate the molecular position with an intensity minimum, such as minimal emission fluxes (MINFLUX) or the more recent raster scanning a minimum of light (RASTMIN), stand out for reaching true molecular resolution. To implement these methods, the phase of the excitation beam needs to be modulated to obtain a focus with a central minimum, i.e., a so-called toroidal or doughnut-shaped focus. In this tutorial, we explain the basis and experimental tricks to generate and optimize such beams, particularly in raster-scanning microscopes.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::882051f36992eed95db334bebc6feabb https://doi.org/10.1364/josab.482413 Zobrazit plný text záznamu