The Relative Biological Effectiveness of Low Doses of 14 MeV Neutrons in Steady-State Murine Spermatogenesis as Determined by Flow Cytometry1
| Autor: | Wolfgang Köhnlein, U. Hacker-Klom, Wolfgang Göhde, H. L. Kronholz |
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| Rok vydání: | 2000 |
| Předmět: | |
| Zdroj: | Radiation Research. 153:734-742 |
| ISSN: | 1938-5404 0033-7587 |
| DOI: | 10.1667/0033-7587(2000)153[0734:trbeol]2.0.co;2 |
| Popis: | Hacker-Klom, U. B., Kohnlein, W., Kronholz, H. L. and Gohde, W. The Relative Biological Effectiveness of Low Doses of 14 MeV Neutrons in Steady-State Murine Spermatogenesis as Determined by Flow Cytometry. The relative biological effectiveness of 14 MeV neutrons in the low-dose range ≤1 Gy has been determined in differentiating and differentiated spermatogonia. Male NMRI mice were exposed to single doses of 2 cGy to 3 Gy of 60Co γ rays or neutrons. The ratios of testicular S-phase cells, 4c primary spermatocytes, and elongated spermatids were quantified by DNA flow cytometry 2 to 70 days after irradiation and were found to decrease. Histological samples and testis weight were analyzed in parallel. Doses of 2–5 cGy neutrons and 10–50 cGy γ rays significantly (P < 0.05) decreased the proportions of S-phase cells, spermatocytes and elongated spermatids at 4, 14 and 28 days postirradiation. For S-phase cells, the biphasic shape of the cell survival curves was described with a D50 of 5 cGy neutrons. T... |
| Databáze: | OpenAIRE |
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