Long-Term Hypoxia Changes Myometrial Responsiveness and Oxytocin Receptors in the Pregnant Ewe: Differential Effects on Longitudinal Versus Circular Smooth Muscle1

Autor:	Hikaru Umezaki, Charles A. Ducsay, T. Imamura, Lubo Zhang, Kanchan M. Kaushal, Malgorzata Mlynarczyk
Rok vydání:	2003
Předmět:	medicine.medical_specialty Myometrium Uterus Cell Biology General Medicine Biology Hypoxia (medical) Oxytocin receptor Endocrinology medicine.anatomical_structure Reproductive Medicine Oxytocin Smooth muscle In utero Internal medicine medicine Gestation medicine.symptom medicine.drug
Zdroj:	Biology of Reproduction. 69:1500-1505
ISSN:	1529-7268 0006-3363
DOI:	10.1095/biolreprod.103.018556
Popis:	Previous studies from our laboratory demonstrated that long-term hypoxia (LTH) altered in vitro contractile responses to oxytocin in full-thickness myometrial strips from pregnant sheep. The present study was designed to determine, first, if the reduced contractile response to oxytocin following LTH is the result of combined effects on longitudinal and circular smooth muscle or if the effect is specific to a single muscle layer and, second, if the reduced contractile response to oxytocin following LTH is caused by changes in oxytocin-receptor protein. Pregnant ewes were maintained at high altitude (3820 m) from Day 30 to Days 137–142 of gestation, when the ewes were killed for collection of myometrial tissue. Tissue was also collected from age-matched, normoxic controls. Longitudinal and circular layers were separated, length-tension curves generated to determine optimal resting tension, and all strips exposed to increasing half-log doses of oxytocin ranging from 10–12 to 10–6.5 M. The expression...
Databáze:	OpenAIRE
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