Enterococcal Infections and Antimicrobial Resistance in a Tertiary Care Hospital, Eastern India
| Autor: | Gopal Krishna Purohit, Nirupama Chayan, Muktikesh Dash, Bimoch Projna Paty, Llalli S Sahu |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Pediatrics
medicine.medical_specialty Computer Networks and Communications Teicoplanin business.industry biochemical phenomena metabolism and nutrition bacterial infections and mycoses Microbiology Ciprofloxacin Penicillin chemistry.chemical_compound Antibiotic resistance chemistry Hardware and Architecture Streptomycin Ampicillin Linezolid medicine Agar diffusion test business Software medicine.drug |
| Zdroj: | Afro-Egyptian Journal of Infectious and Endemic Diseases. 5:255-264 |
| ISSN: | 2090-7184 |
| DOI: | 10.21608/aeji.2015.17846 |
| Popis: | Background and study aim: During last two decades, there has been a world-wide trend in increasing occurrence of entero-coccal infections in the hospitals. The aim of present study was to determine the spectrum of enterococcal infections, speciesprevalence, antimicrobial resistance and characteristics of vancomycin resistant enterococci (VRE) in a tertiary care hospital, Eastern India. Patients and Methods: Between January 2013 and July 2014, 152 Enterococcus species were obtained from clinical samples. Enterococci were identified using standard biochemical tests. Antimicrobial susceptibility was tested by Kirby-Bauer disk diffusion according to Clinical & Laboratory Standards Institute (CLSI) guidelines.VRE agar base was used to screen VRE isolates. Minimum inhibitory concentration (MIC) values of VRE isolates were determined using Epsilometer-test. VRE isolates were also examined by PCR to detect vanA gene. Results: From 1602 clinical samples, 961 (60%) were culture positive and 152(15.8%) enterococcal isolates were obtained. Most common species isolated was E. faecalis (63.8%) followed by E. faecium (35.5%). Majority of enterococcal infections were detected from ICUs and surgical wards and clinically presented as UTIs. Disk diffusion method showed 67.1% were resistant to penicillin, 61.2% ampicillin, 58.5% ciprofloxacin, 46.7% high-level gentamicin, 42. 8% high-level streptomycin, 7.9% teicoplanin and none to linezolid. Twenty (13.2%) enterococcal isolates were vancomycin resistant in VRE screen and disk diffusion method. Epsilometer-test of VRE isolates showed 8 (40%) isolates were resistant and 9 (45%) were intermediately resistant. From 20 VRE isolates, six showed VanA and two VanB phenotypes and all six VanA phenotypes had vanA gene cluster. Conclusion: More accurate and reliable MIC determination tests should be performedin all suspected VRE isolates. Confirmatory PCR is required for identifying resistant gene cluster. |
| Databáze: | OpenAIRE |
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