Fluorescence spectroscopy in biochemistry: teaching basic principles with visual demonstrations

Autor: David M. Jameson, John C. Croney, Robert P. Learmonth
Rok vydání: 2001
Předmět:
Zdroj: Biochemistry and Molecular Biology Education. 29:60-65
ISSN: 1539-3429
1470-8175
DOI: 10.1111/j.1539-3429.2001.tb00071.x
Popis: Although most biochemistry curricula include some treatment of light absorption and spectrophotometry, discussion of fluorescence spectroscopy is generally omitted. This omission is unfortunate given the increasing use of fluorescence in many fields of biochemical research. In this paper we briefly review the principles and applications of fluorescence in biochemical systems, from the viewpoint of teaching fluorescence in undergraduate curricula. Simple practical demonstrations are presented, which clearly demonstrate important concepts yet require minimal specialized equipment.
Databáze: OpenAIRE