Diagnostic test for ruthenium and platinum modification of histidine residues on metalloproteins using diethylpyrocarbonate (DEPC)
| Autor: | Martin P. Jackman, D. G. A. Harshani de Silva, A. Geoffrey Sykes, Peter Osvath, Meng-Chay Lim |
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| Rok vydání: | 1988 |
| Předmět: |
chemistry.chemical_classification
biology Stereochemistry Cytochrome c Azurina chemistry.chemical_element Context (language use) biology.organism_classification Ruthenium Inorganic Chemistry chemistry Materials Chemistry Metalloprotein biology.protein Physical and Theoretical Chemistry Azurin Platinum Histidine |
| Zdroj: | Inorganica Chimica Acta. 153:205-208 |
| ISSN: | 0020-1693 |
| Popis: | The reaction of diethylpyrocarbonate (DEPC) with uncoordinated surface histidine residues on metalloproteins at pH 6.5−7.0 can be readily monitored (∼30 min) by spectrophotometric changes at ∼238 nm (ϵ = 2750 M −1 ). No reaction is observed if prior modification of histidine by attachment of Ru(II) or Pt(II) has been carried out. In the studies with azurin and cytochrome c only one of two histidines readily undergoes DEPC modification, and likewise only one histidine is Ru modified. Thus the reaction can be used as a quick test for histidine availability, and subsequently as a test as to whether modification has been achieved. The specificity of Pt complexes for histidines is less than that of Ru. In a wider context of histidine availability only one of three histidines in Rieske's protein ( N. crassa ) is DEPC modified. |
| Databáze: | OpenAIRE |
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