| Popis: |
Summery. It was developed the complex of the portable biosensors for the non-invasive measures of the chlorophyll fluorescence induction and express control of vegetable loading by some viruses. The first type of biosensor named “Floratest”. It allows to obtaining information about the activity of the photosynthetic process in the tissues which reflects the total state of vegetables. The main advantages of this approach are in its very sensitivity and rapidity. For the control of the viral loading of some vegetables it was developed the portable part of the immune biosensor. In this case as a basis it was used so called laboratory on the crystals Spreeta TSPR1A170100, which produced by the Nomadics, Inc. Spreeta. Reliability of the proposed methods of the express-diagnostics based on the estimation of the induction of the chlorophyll fluorescence in the vegetable flats confirms by the results of the physiology-biochemical analyses: vegetables which are less stable to drought had more high level of chlorophyll fluorescence. Generally it was obtained the conclusion that the determination of the last index by “Floratest” allows to us optimizing the technology of grapes and hot in real time. The results of the analysis of 21 samples with the help of the developed immune biosensor have a good correlation with that obtained by others above mentioned methods. There is necessary to pay attention that the results obtained by the PCR was chosen conditionally as absolute correct since the serological methods have some restrictions which are absent in gene engineering ones. Analysis data are transferred from device to the medical centre or the laboratory by means of radio channel. As radio-transmitter it is used the original unit, which is developed by the company "VD MAIS". Besides the obtained data also the information about the place of the analysis is transferred by means of radio-channel. For this goal it is used GPS-system, which is built in the radio-transmitter. Principle of the operation is based on the transferring all data through the radiochannel (by means of the GSM-technology) directly to the Internet, and then to the server of the medical organization. In any case there are not any doubts that the both developed biosensors may be used at the practice, in particular, for the fulfilment of the screening observations. The together application of both sensors allows to determining not vegetable state only and to reveal the reason of such situation. Moreover, since the principle of work immune biosensor is based on the registration of the bioaffinic reactions it may be considered that this biosensor may help at the diagnostics of many diseases which is stipulated by viruses, bacteria and fungi’s. And it will be perspective at the registration of molecular hybridization. |
