Exploration of structure-activity relationship between IgG1 and IgE binding ability and spatial conformation in ovomucoid with pulsed electric field treatment
| Autor: | Yinli Li, Shuyu Zhang, Songyi Lin, Limin Zhong, Jiang Pengfei, Na Sun |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
Circular dichroism Chemistry Protein primary structure 04 agricultural and veterinary sciences respiratory system Ovomucin 040401 food science 01 natural sciences Protein tertiary structure Fluorescence spectroscopy respiratory tract diseases 0404 agricultural biotechnology immune system diseases 010608 biotechnology Biophysics Fourier transform infrared spectroscopy Protein secondary structure Food Science Egg white |
| Zdroj: | LWT. 141:110891 |
| ISSN: | 0023-6438 |
| DOI: | 10.1016/j.lwt.2021.110891 |
| Popis: | Ovomucin (OVM) is one of the most main allergens in egg white, which limits the application of egg in the field of food processing. In this study, the effect of pulsed electric fields (PEF) on the IgG1 and IgE binding capability and the composition changes of OVM was studied by indirect competitive ELISA, SDS-PAGE, Fourier transform infrared spectroscopy (FTIR), circular dichroism (CD) spectroscopy, contact angle meter, fluorescence spectrophotometer and scanning electron microscopes (SEM). The results suggested that PEF treatment couldsignificantly reduce the IgG1 and IgE binding capacity of OVM, especially under the condition of 30 kV/cm. PEF treatment could cause partial unfolding of the protein, which was attributed to the destruction of the epitope structure that binds to IgG1 and IgE in OVM. Results indicated that PEF treatment significantly changed the primary structure, secondary structure and tertiary structure of the protein. Specifically, the secondary structure of the protein was partially unwound, and hydrophobic amino acids were exposed after PEF treatment. In addition, we observed that the microstructure of the OVM also changed significantly after PEF treatment. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect