Identification of microorganisms for the analysis of images obtained by neutron radiography

Autor:	Claudia Lage, J.D.R. Lopes, V.R. Crispim
Rok vydání:	2001
Předmět:	Radiation Materials science biology Microorganism Neutron imaging Analytical chemistry biology.organism_classification Neutron temperature law.invention chemistry.chemical_compound chemistry Optical microscope Staphylococcus epidermidis law Irradiation CR-39
Zdroj:	Radiation Physics and Chemistry. 61:619-622
ISSN:	0969-806X
DOI:	10.1016/s0969-806x(01)00349-8
Popis:	The main difficulty in identifying infectious microorganisms is the time required to obtain a reliable result, a minimum of 72 h. We propose a reduction to about 5 h through the technique of neutron radiography. Samples containing the bacillus Escherichia coli and the cocci Staphylococcus epidermidis were incubated with B 10 , layered on SSNTD (CR-39) surface and irradiated in the J-9 channel from the Argonauta Reactor (IEN/CNEN) with a flux of thermal neutrons at a rate of 2.2×10 5 n/cm 2 s. Images were observed in an optical microscope after exposure of the plates to chemical development of the latent alpha-tracks. Analysis of the images revealed morphological differences between the species, conferring the technique the perspective to use in microbial diagnosis.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::87b39761d3053d1c181f27608bc2bcf0 https://doi.org/10.1016/s0969-806x(01)00349-8 Zobrazit plný text záznamu Full Text from ScienceDirect