The biodegradation of poly(3-hydroxy-butyrate-co-3-hydroxyvalerate) (PHB/V) and PHB/V-degrading microorganisms in soil

Autor: Shufang Wang, Choichiro Shimasaki, Masami Inoue, Shin Ono, Bang-Hua Zhang, Cunjiang Song
Rok vydání: 2003
Předmět:
Zdroj: Polymers for Advanced Technologies. 14:184-188
ISSN: 1099-1581
1042-7147
Popis: To assess the polyhydroxyalkanoate (PHA)-biodegrading capacity of soil, numbers of aerobic poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHB/V)-degrading microorganisms (degraders) were estimated by the film-MPN method. The numbers of PHB/V degraders were estimated to be 4.3 × 105 per gram of dry garden soil, 5.06 × 105 per gram of dry paddy-field soil, and 3.87 × 105 per gram of dry river-bank soil. The degradations of PHB/V in suspensions of the three kinds of soil were investigated. It was found that the PHB/V-biodegrading capacity of the soil increased as the number of PHB/V degraders in the soil increased. The relationship between weight loss of PHB/V and proliferation rate of PHB/V degraders in garden soil suspension was investigated. The effect of glucose on the biodegradation of PHB/V in garden soil was also studied. The weight loss after one week in garden soil suspension supplemented with 20 mM of glucose (GSS-20G) was 2.60%, which was lower than that in garden soil suspension (GSS) (7.14%). After five weeks, the weight loss had increased to 24.97% in GSS-20G but only to 18.26% in GSS. The results showed that glucose played important roles in inhibition and acceleration of different biodegrading phases and finally accelerated the PHB/V biodegradation in soil suspension. Copyright © 2003 John Wiley & Sons, Ltd.
Databáze: OpenAIRE
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