Fractionation of nitrogenous compounds from water extracts of doenjang and their physiological activities

Autor:	Jae Cherl, Hyun A. Lee, Hyesook Lee, Sang Hyun Lee, Hyun Do Jeong, Jae Han Jung
Rok vydání:	2014
Předmět:	chemistry.chemical_classification Chromatography Molecular mass Lipopolysaccharide Peptide Fractionation Applied Microbiology and Biotechnology Ultrafiltration (renal) chemistry.chemical_compound Enzyme chemistry Biochemistry IC50 Ace inhibition Food Science Biotechnology
Zdroj:	Food Science and Biotechnology. 23:523-529
ISSN:	2092-6456 1226-7708
DOI:	10.1007/s10068-014-0072-x
Popis:	Nitrogenous compounds were separated from a water extract of doenjang (WED), and the distribution of their molecular weights, and anti-inflammatory and angiotensin-I converting enzyme (ACE) inhibitory activities were investigated. WED was fractionated by ultrafiltration into 5 individual groups. The nitrogenous compound content in the 5 fractions were 1,062.1, 34.5, 24.2, and 29.9 mg% for WED I, WED II, WED III, and WED V, respectively. ACE inhibitory activity was widely observed in all the fractions, which suggest that many ACE inhibitory peptides with various molecular weights were present. However, the most potent ACE inhibition was observed in WED I with an IC50 value of 116.90±2.25 μg/mL. Potent inhibitory effects on the production of inflammatory mediators were observed in the extracts of doenjang with molecular weights >1 kDa (WED II, WED III, and WED V), as demonstrated by the inhibition of lipopolysaccharide induced NO production in macrophages.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::8798e20bcd6c7162ccbbf45bef163bff https://doi.org/10.1007/s10068-014-0072-x Zobrazit plný text záznamu Full text from SpringerLink