Molecular variability of a Minnesota population ofPhoma medicaginisvar.medicaginis, the causal agent of spring black stem and leaf spot of alfalfa
| Autor: | Claudia V. Castell-Miller, Deborah A. Samac, Nichole R. O'Neill, Liane R. Gale, Les J. Szabo |
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| Rok vydání: | 2008 |
| Předmět: |
education.field_of_study
biology fungi Population food and beverages Population genetics Plant Science Fungi imperfecti biology.organism_classification Botany Temperate climate Amplified fragment length polymorphism Medicago sativa Internal transcribed spacer education Agronomy and Crop Science Ribosomal DNA |
| Zdroj: | Canadian Journal of Plant Pathology. 30:85-96 |
| ISSN: | 1715-2992 0706-0661 |
| DOI: | 10.1080/07060660809507499 |
| Popis: | Spring black stem and leaf spot of alfalfa (Medicago sativa) caused by Phoma medicaginis var. medicaginis is an important disease of alfalfa in temperate regions of the United States, causing yield losses and decreasing forage quality. Isolates of the fungus vary in morphological and cultural characteristics, as well as aggressiveness on alfalfa. The sequence of the internal transcribed spacer (ITS) regions of nuclear ribosomal DNA (rDNA) and amplified fragment length polymorphism (AFLP) fingerprinting were used to characterize the diversity of P. medicaginis var. medicaginis isolates collected from leaves and stems of alfalfa plants from northern and southern areas of Minnesota. Sequences of the ITS1-5.8S-ITS2 regions of the rDNA repeats from 14 isolates were highly conserved. AFLP analysis of 121 isolates with eight primer pair combinations revealed a moderate amount of variation as measured by the Dice coefficient (55%) and a gene diversity index of H = 0.23. Molecular polymorphisms were not correlated... |
| Databáze: | OpenAIRE |
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