Activation of the neutrophil respiratory burst by plasma from periodontitis patients is mediated by pro-inflammatory cytokines

Autor:	Christopher R. Dunston, Helen J. Wright, Iain L. C. Chapple, Irundika H.K. Dias, John B. Matthews, Helen R. Griffiths
Rok vydání:	2010
Předmět:	biology business.industry medicine.medical_treatment Albumin Inflammation Granulocyte Proinflammatory cytokine Respiratory burst Cytokine medicine.anatomical_structure Blood plasma Immunology medicine biology.protein Periodontics medicine.symptom Antibody business
Zdroj:	Journal of Clinical Periodontology. 38:1-7
ISSN:	0303-6979
Popis:	Aim: To determine the effect of periodontitis patients' plasma on the neutrophil oxidative burst and the role of albumin, immunoglobulins (Igs) and cytokines. Materials and Methods: Plasma was collected from chronic periodontitis patients (n=11) and periodontally healthy controls (n=11) and used with/without depletion of albumin and Ig or antibody neutralization of IL-8, GM-CSF or IFN-a to prime/stimulate peripheral blood neutrophils, isolated from healthy volunteers. The respiratory burst was measured by lucigenin-dependent chemiluminescence. Plasma cytokine levels were determined by ELISA. Results: Plasmas from patients were significantly more effective in both directly stimulating neutrophil superoxide production and priming for subsequent formyl-met-leu-phe (fMLP)-stimulated superoxide production than plasmas from healthy controls (p
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::87941b9e01627346f07cea5c2404c2ce https://doi.org/10.1111/j.1600-051x.2010.01628.x Zobrazit plný text záznamu Plný text