Evaluation of genetic diversity in 19 Glycine max (L.) Merr. accessions included in the Czech National Collection of soybean genotypes

Autor: M. Kadlec, Jana Raddová, Miroslav Baránek, Miroslav Vachůn, Miroslav Pidra
Rok vydání: 2002
Předmět:
Zdroj: Czech Journal of Genetics and Plant Breeding. 38:69-74
ISSN: 1805-9325
1212-1975
Popis: The random amplified polymorphic DNA (RAPD) technique was used to evaluate both genetic diversity among 19 soybean accessions included in the Czech National Collection of Soybean Genotypes and their potential as a new source of genetic variations for soybean breeding programs. Only 22 of all the 40 random primers used in RAPD reactions showed poly- morphism acceptable for an effective characterisation of these accessions. Altogether 122 highly reproducible RAPD fragments were generated, 55 of them were polymorphic (46%). However, because of the previously observed low degree of RAPD poly- morphism in the case of Glycine max, fragments with low level of informativeness were evaluated, too. Presented results enable the selection of genetically distinct individuals. Such information may be useful to breeders willing to use genetically divers e introductions in soybean improvement process.
Databáze: OpenAIRE