Use of High-Performance Size-Exclusion Chromatography for Characterization of Amylose Isolated from Diverse Botanical Sources

Autor:	Francisco Hernández-Centeno, Paul Baruk Zamudio-Flores, René Salgado-Delgado, Juan G. Torruco-Uco, Juan Manuel Tirado-Gallegos, Carolina E. Chávez-Murillo
Rok vydání:	2015
Předmět:	Molar mass Chromatography Dispersity Size-exclusion chromatography Pullulan 04 agricultural and veterinary sciences 040401 food science Standard curve chemistry.chemical_compound 0404 agricultural biotechnology chemistry Amylose Amylopectin Molar mass distribution Food Science
Zdroj:	International Journal of Food Properties. 19:1362-1369
ISSN:	1532-2386 1094-2912
DOI:	10.1080/10942912.2015.1080269
Popis:	Amyloses were isolated from diverse botanical sources (apple, mango, maize, and potato), and they were studied in their molecular characteristics (amylose content, molar mass, and molecular weight) using high-performance size-exclusion chromatography as a repetitive and faster protocol. The amylose purity ranged between 85.6–92.6 %, in agreement with the λmax values (601–610 nm), showing that some impurities with molecules of higher molar mass (amylopectin) were present. The standard curve of pullulan showed a high regression coefficient (0.998) inside of the limits of molar mass of amylose. Chromatograms of amylose showed the presence of components of high molar mass with a principal peak that corresponds to amylose. Molar mass of amylose ranged between 1.2 and 8.5 × 105 g/mol with polydispersity values between 1.3–4.1, indicating a narrow range of molar mass distribution of the amyloses analyzed. The high-performance size-exclusion chromatography coupled with a refractive index methodology used in this ...
Databáze:	OpenAIRE
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