Aplastic anemia: evidence for dysfunctional bone marrow progenitor cells and the corrective effect of granulocyte colony-stimulating factor in vitro
| Autor: | Edward C. Gordon-Smith, Richard Atkinson, Frances M. Gibson, John Scopes, Sarah E. Ball, S. Daly |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
medicine.medical_specialty
Immunology Stem cell factor Cell Biology Hematology Biology medicine.disease Biochemistry Granulocyte colony-stimulating factor medicine.anatomical_structure Endocrinology Granulocyte macrophage colony-stimulating factor Internal medicine medicine Bone marrow Stem cell Aplastic anemia Antigen-presenting cell Interleukin 3 medicine.drug |
| Zdroj: | Blood. 87:3179-3185 |
| ISSN: | 1528-0020 0006-4971 |
| Popis: | We investigated the effects of granulocyte-macrophage colony- stimulating factor, interleukin-3, stem cell factor, interleukin-6, and granulocyte colony-stimulating factor (G-CSF) alone, and in combination, on the clonogenic potential of normal and aplastic anemia (AA) bone marrow mononuclear cells (BMMC and CD34+ cells. AA BMMC consistently produced a significantly lower absolute number of colonies than normal, but, when account was taken of the reduced proportion of CD34+ cells in AA BM, there was no significant difference in terms of cloning efficiency (CE). However, when removed from the influence of accessory cells, the CE of AA CD34+ cells decreased significantly more than normal, indicating a defect in their function, either in terms of dependence on accessory cell-derived factors or susceptibility to cell damage when sorted. Of the factors studied, G-CSF had the most significant effect on the response of CD34+ cells from both groups when removed from their accessory cells. This was particularly true for AA CD34+ cells, whose response to cytokine stimuli containing G-CSF enabled them to match the response of normal CD34+ cells. |
| Databáze: | OpenAIRE |
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