The use of vibratome sections of cereal spikelets to study anther development and meiosis

Autor:	Graham Moore, Peter Shaw, Alison F. Beven, Luis Aragón-Alcaide
Rok vydání:	1998
Předmět:	Tapetum medicine.diagnostic_test fungi food and beverages Cell Biology Plant Science Meiocyte Biology law.invention Cell biology Vibratome Meiosis Confocal microscopy law Botany Genetics Homologous chromosome medicine Telophase Fluorescence in situ hybridization
Zdroj:	The Plant Journal. 14:503-508
ISSN:	0960-7412
DOI:	10.1046/j.1365-313x.1998.00148.x
Popis:	Summary We have developed a method using vibratome sectioning coupled with confocal microscopy to analyse pre-meiotic and meiotic events in developing wheat anthers. This report describes the application of fluorescence in situ hybridization to three-dimensionally preserved tissue sections from intact florets. We show that excellent imaging of all the stages of meiosis up to telophase I can be achieved, and show centromere positions and pre-meiotic homologous pairing both in meiocytes and tapetum cells. These clear results using intact tissue sections are contrasted with conventional spreading methods for analysis of meiosis in plants, which we show can produce artefactual or uninterpretable results.
Databáze:	OpenAIRE
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