Molecular diagnosis of phytoplasma transmission from zygotic embryos to in vitro regenerated plants of coconut palm (Cocos nucifera L.)
| Autor: | Koffi Yoboue, Arocha Rosete Yaima, Wentoin Alimata Marie Pierre Daramcoum, Kan Modeste Kouassi, Saraka Didier Martial Yao, Eric-Blanchard Zadjéhi Koffi, Assanvo Simon-Pierre N’guetta, Konan Jean Louis Konan, Claude Ghislaine Kouadjo, Kouadio Kan Ghislain Koffi, Edmond Koffi |
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| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine biology food and beverages Embryo Ribosomal RNA biology.organism_classification 01 natural sciences Applied Microbiology and Biotechnology law.invention Plantlet 03 medical and health sciences Horticulture 030104 developmental biology Cocos nucifera Phytoplasma law Genetics Phloem Agronomy and Crop Science Molecular Biology Polymerase chain reaction Lethal yellowing 010606 plant biology & botany Biotechnology |
| Zdroj: | African Journal of Biotechnology. 17:862-869 |
| ISSN: | 1684-5315 |
| Popis: | The aim of this study was to investigate the transmission of the lethal yellowing disease (LYD) of coconut tree caused by a phytoplasma from the zygotic embryo to the regenerated plantlet in vitro. From a total of 30 trees, 150 mature coconut nuts where harvested. These nuts were used to extract 150 zygotic embryos. From this package, 96 zygotic embryos were used to regenerate 96 young coconut seedlings in vitro and the 54 others were used to extract total DNA. From the stem of the 30 palms at the stage 1 of the LYD, phloem sample were also collected. From the regenerated in vitro-plantlets at 6 months age, leaf sample were collected. From the molecular diagnosis by PCR, 80% of the phloem samples carried the 16S rRNA gene of the phytoplasma responsible for LYD. All the zygotic embryos and in vitro-plantlets regenerated were healthy. So, coconut zygotic embryos can be used for the safe exchange of genetic material regarding lethal yellowing disease. The regenerated in vitro plantlet are free of disease. Key words: Coconut, phytoplasma, transmission, in vitro. |
| Databáze: | OpenAIRE |
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