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Using a PCR-based approach we have isolated and characterized a putative plant resistance gene from Triticum turgidum whose corresponding resistance gene has been identified in Triticum aestivum . Furthermore, the partial sequences of four new resistance gene analogs (RGA), were used to study their expression profiles in leaves and in roots of two different varieties of T. turgidum . The analysis of the conserved motifs clearly indicates that the sequences we have isolated belong to the NBS-LRR class of plant resistance genes. Expression studies in healthy plants, using the varieties Creso and Latino of T. turgidum , indicate different localizations of RGA transcripts and suggest that they function in the general mechanism of plant surveillance against pathogens. |
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