Screening for Urease-Producing Bacteria from Limestone Caves of Sarawak

Autor:	Phua Ye Li, Peter Morin Nissom, Nurnajwani Senian, Irine Runnie Henry Ginjom, Dominic Ong Ek Leong, Armstrong Ighodalo Omoregie, Ngu Lock Hei
Rok vydání:	2016
Předmět:	chemistry.chemical_classification biology Urease Applied Mathematics General Mathematics Microorganism biology.organism_classification Enrichment culture Sporosarcina pasteurii Microbiology chemistry.chemical_compound Enzyme chemistry Catalase biology.protein Urea Bacteria
Zdroj:	Borneo Journal of Resource Science and Technology. 6:37-45
ISSN:	0128-2972 2229-9769
Popis:	Urease is a key enzyme in the chemical reaction of microorganism and has been found to be associated withcalcification, which is essential in microbially induced calcite precipitation (MICP) process. Three bacterialisolates (designated as LPB19, TSB31 and TSB12) were among twenty-eight bacteria that were isolated fromsamples collected from Sarawak limestone caves using the enrichment culture technique. Isolates LPB19, TSB31and TSB12 were selected based on their quick urease production when compared to other isolates. Phenotypiccharacteristics indicate all three bacterial strains are gram-positive, rod-shaped, motile, catalase and oxidasepositive. Urease activity of the bacterial isolates were measured through changes in conductivity in the absence ofcalcium ions. The bacterial isolates (LPB19, TSB12 and TSB31) showed urease activity of 16.14, 12.45 and 11.41mM urea hydrolysed/min respectively. The current work suggested that these isolates serves as constitutiveproducers of urease, potentially useful in inducing calcite precipitates.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::874a13ca494274306de930473268badf https://doi.org/10.33736/bjrst.213.2016 Zobrazit plný text záznamu