The lipolytic degradation of highly structured cubic micellar nanoparticles of soy phosphatidylcholine and glycerol dioleate by phospholipase A 2 and triacylglycerol lipase
| Autor: | Justas Barauskas, Tommy Nylander, Fredrik Tiberg, Maria Wadsäter |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Phospholipase A Chromatography 030102 biochemistry & molecular biology biology Organic Chemistry Triacylglycerol lipase Phospholipid 02 engineering and technology Cell Biology 021001 nanoscience & nanotechnology Biochemistry 03 medical and health sciences chemistry.chemical_compound Hydrolysis Phospholipase A2 Lamellar phase chemistry Phosphatidylcholine biology.protein lipids (amino acids peptides and proteins) Diglyceride 0210 nano-technology Molecular Biology |
| Zdroj: | Chemistry and Physics of Lipids. 211:86-92 |
| ISSN: | 0009-3084 |
| DOI: | 10.1016/j.chemphyslip.2017.11.011 |
| Popis: | The effects of different lipolytic enzymes on the structure of lipid liquid crystalline nano-particles (LCNP) have been investigated by cryogenic transmission electron microscopy (cryo-TEM) and synchrotron small angle X-ray diffraction (SAXD). Here we used highly structured cubic micellar (Fd3m) nanoparticles of 50/50 (wt%/wt%) soy phosphatidyl choline (SPC)/glycerol dioleate (GDO) as substrate. Two types of lipolytic enzymes were used, phospholipase A2 (PLA2) that catalyses degradation of the phospholipid component, SPC, and porcine pancreatic triacylglycerol lipase (TGL) that facilitate the hydrolysis of the diglyceride, GDO. Evolution of the structure was found to be very different and linked to specificity of the two types of enzymes. PLA2, which hydrolyses the lamellar forming component, SPC, induces a reversed micellar lipid phase, while TGL which hydrolysis the reverse phase forming compound, GDO, induces a lamellar phase. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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