Optimized Assay for Hydrogen Peroxide Determination in Plant Tissue Using Potassium Iodide

Autor:	Huguette Sallanon, Félicie Lopez-Lauri, Laurent Urban, Sanders Junglee
Rok vydání:	2014
Předmět:	Antioxidant Chromatography medicine.medical_treatment Extraction (chemistry) Inorganic chemistry chemistry.chemical_element One-Step Iodine Plant tissue Buffer (optical fiber) Standard curve Psychiatry and Mental health chemistry.chemical_compound chemistry medicine Hydrogen peroxide
Zdroj:	American Journal of Analytical Chemistry. :730-736
ISSN:	2156-8278 2156-8251
DOI:	10.4236/ajac.2014.511081
Popis:	Here, we present an optimization of colorimetric determination of hydrogen peroxide content in plants using potassium iodide. Our method is based on a one step buffer (extraction and reaction) for the determination of H2O2 in different plant tissues and overcomes interference of soluble antioxidant and color background. A particular attention is paid to buffer pH shown to be tissue dependent. With this inexpensive microplate method, it is possible to analyze 12 experimental samples in about 45 min all in triplicates, with blanks, controls and standard curve.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::8741f5a3b06d89b5be366e7943b46df6 https://doi.org/10.4236/ajac.2014.511081 Zobrazit plný text záznamu