Efficacy of extracts and iridoid glucosides from Pentas lanceolata on humoral and cell-mediated immune response of viral vaccine

Autor: Heba-tollah M. Sweelam, Mohamed-Elamir F. Hegazy, Mounir M. El-Safty, Mahmoud M. Gabr, Howaida I. Abd-Alla, Tarik A. Mohamed
Rok vydání: 2017
Předmět:
Zdroj: Medicinal Chemistry Research. 26:2196-2204
ISSN: 1554-8120
1054-2523
DOI: 10.1007/s00044-017-1935-5
Popis: The 80% aqueous methanol extract of Pentas lanceolata (Rubiaceae) was partitioned with n-hexane, ether, ethyl acetate extract, and n-butanol extract. The immunomodulatory role of the five extracts on humoral and cell-mediated immune response of viral vaccine in specific pathogen-free chicken vaccinated against Newcastle disease virus was carried out. The active fractions, n-butanol, was fractioned by silica-column chromatography and active sub-fraction were separated using reverse phase-high performance liquid chromatography. Two new iridoids, namely, 13R-methoxy-epi-gaertneroside 2 and 13S-methoxy-epi-gaertneroside 3, and three known, 13R-epi-gaertneroside 1, a mixture of E-uenfoside 4 and Z-uenfoside 5, were isolated from the active sub-fractions. Their chemical structures were achieved by the extensive 1D and 2D nuclear magnetic resonance (1H and 13C, correlation spectroscopy, heteronuclear multiple quantum correlation, and heteronuclear multiple bond correlation), IR as well as HREIMS spectral data. Stimulation of both seroresponses was observed, especially those of ethyl acetate extract, n-butanol extract, and compound 1. Remarkable effective increase of the antibody titers; lymphocyte and macrophage cells in blood were recorded. The study suggested that the possible role was played by P. lanceolata aerial parts n-butanol, ethyl acetate, and three iridoids 1, 4, and 5 in immunity (humoral and cellular) against Newcastle disease which is severely endangering out the poultry industry by causing economic losses.
Databáze: OpenAIRE
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