| Popis: |
Amino acids emerged in various biochemical cycles in vivo, affecting the performance and carcass characteristics of broilers. In this study, the contents of dietary lysine and their effects on carcass performance of broiler were investigated. Experimental results showed that deficient dietary lysine significantly reduced live weight, carcass weight, eviscerated yield with gihlet (EYG) and eviscerated yield (EY) of broilers. Whereas, the percentage of slaughter yield, the percentage of eviscerated yield (PEY) and the percentage of eviscerated yield with giblet (PEG) were slightly affected. Many CpG sites were found at the myostatin exon 1 region (nucleotides 2360-2980 bp). At the myostatin exon 1 region where CpG sites enriched (nucleotides 2360-2980 bp), we found significantly lower methylation rate of 18% in low-lysine group of 55-day-old broilers, compared with methylation rate of 87% in control group, which suggested the deficient dietary lysine may lead to demethylation of their original methylated sites. In muscle tissues, the exon1 hypermethylation status of myostatin gene was detected, which was negative correlated with the expression of this gene. These results suggested that methylation of this gene was a dynamic process, which plays a dominant role in regulation of gene expression for development of individuals. |
