Analyses of Various Fatty Acid Esters by Reverse Phase High-Performance Liquid Chromatography

Autor:	Satoshi Nakasato, Hirohumi Hirata, Katsuhiko Higuchi
Rok vydání:	1984
Předmět:	chemistry.chemical_classification chemistry.chemical_compound Column chromatography Chromatography Volume (thermodynamics) chemistry Phase (matter) Fatty acid chemistry.chemical_element Alcohol Reversed-phase chromatography High-performance liquid chromatography Carbon
Zdroj:	Journal of Japan Oil Chemists' Society. 33:290-293
ISSN:	1884-2003
DOI:	10.5650/jos1956.33.290
Popis:	Reverse phase HPLC of various fatty acid esters has been studied using 050% H2O-MeOH as eluent. The esters were detected at 200210nm. The plots of log (corrected retention volume : V) vs. carbon numbers (CNs) of acid and alcohol residues in saturated esters gave good linear relationships. The slopes (Δlog V/ΔCN) of log V vs. CN of esters plots increased linearly with increasing the water content in eluent. Plots of area ratio vs. weight ratio of unsaturated methyl ester/12 : 0-Me gave good linear relationships, demonstrating a possibility of quantitation of fatty acid esters by reverse phase HPLC.
Databáze:	OpenAIRE
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