Phytohemagglutinin stimulation of human lymphocytes
| Autor: | Inna Saporoschetz, Peter Polgar, Sidney R. Cooperband, John A. Mannick, Alexander M. Rutenburg, Hwakyu Kim, Arthur H. Glasgow |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
medicine.medical_specialty
Lymphocyte Immunology Adenylate kinase chemical and pharmacologic phenomena Stimulation Radioimmunoassay Biology Cyclase Pathology and Forensic Medicine medicine.anatomical_structure Endocrinology Internal medicine medicine Immunology and Allergy Phosphodiesterase inhibitor Cyclase activity Intracellular |
| Zdroj: | Clinical Immunology and Immunopathology. 3:353-362 |
| ISSN: | 0090-1229 |
| Popis: | Cyclic AMP content and adenylate cyclase activity were determined in human lymphocytes stimulated to proliferate by phytohemagglutinin (PHA). Both commercial (Difco PHA-M) and purified PHA were used at optimal stimulatory concentrations as confirmed by incorporation of [14C]leucine. Cyclic AMP levels were measured by radioimmunoassay. Exposure of lymphocytes to PHA-M alone yielded no significant change in intracellular cAMP levels; however, in the presence of the phosphodiesterase inhibitor, caffeine, PHA-treated cells had lower levels of cAMP than the caffeine controls. That adenylate cyclase remained active in these experiments was shown by marked increases in lymphocyte cAMP levels following exposure to prostaglandin E1 (PGE1). Stimulatory concentrations of PHA-M or purified PHA also failed to induce adenylate cyclase activity in lymphocyte particulate preparations whereas PGE1 or NaF produced significant increases in enzyme activity. Moreover, PGE1, at concentrations which caused a prompt and significant rise in adenylate cyclase activity and in intracellular cAMP levels, inhibited lymphocyte stimulation by PHA. |
| Databáze: | OpenAIRE |
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