| Autor: |
Nilmara de Oliveira Alves, Olivier Boulard, Amaury Vaysse, Guillaume Dalmasso, Darja Nikitina, Richard Ruez, Thierry Pedron, Emma Bergstein, Pierre Sauvanet, Diane Letourneur, Catherine Godfraind, Imène Najjar, Emmanuel Lemichez, Juan Lucio Iovanna, Denis Mestivier, Nicolas Barnich, Philippe Sansonetti, Christophe Malabat, Marc Monot, Sean Kennedy, Amel Mettouchi, Richard Bonnet, Iradj Sobhani, Mathias Chamaillard |
| Rok vydání: |
2023 |
| Popis: |
The cellular heterogeneity within a variety of solid tumors is influenced by several type of tumor-associated bacteria through yet poorly understood mechanisms. Unbiased genomic analysis revealed that right-sided colorectal tumors with poorer prognosis are preferentially populated with species related toEscherichia coli, including strains that are producing colibactin. By applying metabolomic profilingin situ, the presence of colibactin-producingEscherichia coli(CoPEC) was identified to establish a high-glycerophospholipid microenvironment within right-sided colorectal tumors that are bearing mutations in either APC or KRAS. Using spatial approaches, we revealed that bacterial microniches are poorly infiltrated with CD8+T cells. The aforementioned alterations in lipid metabolism negatively correlated with immunomodulatory and neurotrophic factors among which the human regenerating family member 3 alpha gene (REG3A). Accordingly, the loss of its ortholog enhances growth at the proximal colon of tumors with a microenvironment that impedes immune surveillance and shares similarities to human right-sided colorectal tumors. This work clarifies how the presence of colibactin-producing bacteria may locally establish tumor heterogeneity for lipid metabolism to promote cancer progression and provide unique insights both for therapeutic intervention and enabling basic research into the mechanisms of microbiota-host interaction.STATEMENT OF SIGNIFICANCEApplyingin situmetabolic imaging to patients’ tumors revealed that the accumulation of colibactin-producing bacteria that locally creates a potential vulnerability to cancer treatments through lipid metabolic reprogramming of tumor cells. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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