Polar adhesion of Treponema denticola on wettability gradient surfaces
| Autor: | D A Grove, Richard P. Ellen, Maude Wikström, Hans Elwing, Meja Song |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
biology
Treponema denticola Surfaces and Interfaces General Medicine Adhesion biology.organism_classification Staining chemistry.chemical_compound Colloid and Surface Chemistry Biochemistry chemistry Fluorescence microscope biology.protein Biophysics Propidium iodide Wetting Physical and Theoretical Chemistry Bovine serum albumin Bacteria Biotechnology |
| Zdroj: | Colloids and Surfaces B: Biointerfaces. 11:177-186 |
| ISSN: | 0927-7765 |
| DOI: | 10.1016/s0927-7765(98)00034-4 |
| Popis: | The effects of surface wettability and protein conditioning on adhesion orientation and viability of Treponema denticola were studied using wettability gradient surfaces containing hydrophobic –Si(CH 3 ) 2 bound to silicon or glass. Adhesion and viability were also compared using hydrophobic and hydrophilic plastics used in bacteriology and tissue culture, respectively. Adhesion to gradients on opaque silicon surfaces was quantified by fluorescence microscopy, and the percentage of bacteria adherent in polar orientation to gradients on transparent surfaces was determined by darkfield microscopy. The experiments were repeated with fibronectin (Fn), fibrinogen and bovine serum albumin conditioned surfaces. Determination of viability was attempted by using fluorescent staining with a carboxyfluorescein diacetate (CFDA) and propidium iodide (PI) mixture as well as scanning the surfaces for microcolonies when the adherent bacteria were placed under growth-promoting conditions. More bacteria bound to hydrophobic sectors of the gradient than to the hydrophilic sector. Virtually all the treponemes on the hydrophobic sector were oriented flat on the surface. In samples rinsed conventionally with buffer, they were deformed, and relatively few stained with CFDA or survived under growth-promoting conditions. Many of the cells adherent to hydrophilic surfaces were tip-oriented, and they retained typical treponemal morphology. Within limitations of the method, the percentage staining with CFDA and excluding PI was higher than on hydrophobic surfaces. More microcolonies were detected on hydrophilic surfaces under growing conditions. Conditioning the gradient surfaces with proteins nearly equalized the number of adherent bacteria among the sectors. Fn conditioning promoted time-dependent polar orientation and survival of bacteria with typical treponemal morphology and CFDA staining. Many more bacteria survived on hydrophobic surfaces that were continuously immersed in buffer and not exposed to the atmosphere during rinsing. Directly staining adherent bacteria with the fluorescent vitality indicators was too unreliable for determining whether individual tip-oriented T. denticola cells survived preferentially. Polar orientation is a phenotype common in treponemal adhesion to biological and wettable inanimate surfaces preconditioned with specific proteins, but direct evidence that it is a major factor for maintaining viability is elusive. |
| Databáze: | OpenAIRE |
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