Substrate specificity of acyl-lipid Δ9-desaturase from Prochlorothrix hollandica cyanobacterium producing myristoleic acid
| Autor: | Dmitry A. Los, S. S. Lapina, Maria A. Sinetova, A. A. Userbaeva, Bolatkhan K. Zayadan, V. P. Pchelkin, I. P. Maslova, Kirill S. Mironov, A. Yu. Starikov |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine ATP synthase Stereochemistry Membrane lipids Plant physiology Substrate (chemistry) Plant Science Biology medicine.disease_cause 01 natural sciences 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology Biochemistry chemistry Chlorophyll Myristoleic acid medicine biology.protein lipids (amino acids peptides and proteins) Heterologous expression Escherichia coli 010606 plant biology & botany |
| Zdroj: | Russian Journal of Plant Physiology. 64:560-565 |
| ISSN: | 1608-3407 1021-4437 |
| Popis: | Chlorophyll b-containing cyanobacterium Prochlorothrix hollandica is characterized by a high content of esterified fatty acids (FA) with 14 and 16 carbon atoms in the membrane lipids. Depending on the conditions of cultivation, the relative amount of myristic (C14:0) and myristoleic (C14:1) acids can reach 35%, and palmitic (С16:0) and palmitoleic (С16:1) acids can reach 60% of the sum of all fatty acids in cells. Monounsaturated FAs are represented by C14:1, and C16:1 with an olefinic bond presumably located in the Δ9 position. We cloned the gene of acyl-lipid Δ9-desaturase, desC1, from Prochlorothrix hollandica and characterized its specificity to the length of the substrate using the heterologous expression in Escherichia coli cells adding C14:0 or stearic (C18:0) acids as exogenous substrates. The results show that DesC1 Δ9 desaturase generates olefinic bonds in the FAs with a length of 14 to 18 carbon atoms with an approximately equal efficiency. This indicates that the length of the FA chain in P. hollandica is determined by the activity of the FA synthase, and the chain is desaturated at the Δ9 position nonspecifically relatively to its length. |
| Databáze: | OpenAIRE |
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