Comparative Study of beta-Glucosidase from Cotyledons and Fruits of Cucumber, Cucumis sativus
| Autor: | David M. Pharr, J. D. McCREIGHT, H. N. Sox, R. L. Lower |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
chemistry.chemical_classification
food.ingredient Physiology food and beverages Heat stability Cell Biology Plant Science General Medicine Biology biology.organism_classification Michaelis–Menten kinetics Horticulture food Enzyme chemistry Botany Genetics Competitive inhibitor β glucosidase Cucumis Cotyledon |
| Zdroj: | Physiologia Plantarum. 37:17-22 |
| ISSN: | 1399-3054 0031-9317 |
| DOI: | 10.1111/j.1399-3054.1976.tb01866.x |
| Popis: | This study was conducted to compare the β-glucosidase of cotyledons and fruits of Cucumis sativus L. cv. Chipper. The concentration of the enzyme was followed throughout the growth period of each organ. The greatest concentration of the enzyme did not correspond with the most rapid period of growth. Each enzyme was characterized kinetically. The Michaelis constant of the cotyledon β-glucosidase for p-NO2-phenyl-β-D-gluco-pyranoside was 1.57 mM, and was 0.35 mM for the fruit enzyme. The enzymes from the two sources also differed in affinity for glucono-1,5-lactone, a competitive inhibitor of β-glucosidases, susceptibility to inhibition by saccharides, and heat stability. The two organs apparently contain different forms of β-glucosidase. |
| Databáze: | OpenAIRE |
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