Myocardial reperfusion injury
| Autor: | Steven E. Calvano, Wilson Ko, Anthony C. Antonacci, Karl H. Krieger, W. Douglas Lazenby, Arthur S. Hawes, John A. Zelano, Yong T. Shin, O. Wayne Isom |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Pulmonary and Respiratory Medicine
medicine.medical_specialty Platelet-activating factor business.industry Ischemia Infarction Radioimmunoassay Anterior Descending Coronary Artery medicine.disease chemistry.chemical_compound Endocrinology chemistry Coronary occlusion Internal medicine Anesthesia medicine Surgery Cardiology and Cardiovascular Medicine Hydrogen peroxide business Coronary sinus |
| Zdroj: | The Journal of Thoracic and Cardiovascular Surgery. 102:297-308 |
| ISSN: | 0022-5223 |
| DOI: | 10.1016/s0022-5223(19)36563-8 |
| Popis: | To study the roles of platelet-activating factor, polymorphonuclear leukocytes, and oxygen free radicals in myocardial reperfusion injury, we subjected 10 sheep to 90 minutes of mid-left anterior descending coronary artery followed by 6 hours of reperfusion. Stainings with gentian violet and tetratriphenyl ammonium chloride demonstrated 20% ± 3% of the left ventricular mass at risk for ischemia, of which 75% ± 10% underwent infarction. Coronary sinus blood was assayed for platelet-activating factor and neutrophil hydrogen peroxide production before and during coronary occlusion and during reperfusion. Platelet-activating factor was isolated by column chromatography and lipid extraction and quantified by radioimmunoassay. Neutrophil hydrogen peroxide production was measured by a 2′,7′-dichlorofluorescein flow-cytometric assay. Platelet-activating factor was elevated to 899 ± 210 pg/ml at 15 minutes of reperfusion, compared with the preocclusion level of 271 ± 55 pg/ml and coronary occlusion level of 359 ± 64 pg/ml (p |
| Databáze: | OpenAIRE |
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