Production of Toxoplasma gondii Recombinant Antigens in Genome-Edited Escherichia coli
| Autor: | S. Amaral, J. Bertran, D. Goti, A. Redondo, D. Wood, J. Ferré |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0301 basic medicine Clone (cell biology) Biology medicine.disease_cause 01 natural sciences Applied Microbiology and Biotechnology Biochemistry law.invention 03 medical and health sciences Antigen law 010608 biotechnology parasitic diseases medicine Escherichia coli Toxoplasma gondii biology.organism_classification medicine.disease Fusion protein Virology Toxoplasmosis 030104 developmental biology Recombinant DNA biology.protein Antibody |
| Zdroj: | Applied Biochemistry and Microbiology. 57:152-160 |
| ISSN: | 1608-3024 0003-6838 |
| Popis: | Toxoplasmosis is a widespread zoonosis with an impact on immunocompromised people and critical in pregnant women because of its transmission to the fetus. Recombinant Toxoplasma gondii antigens produced in Escherichia coli are useful for antibody detection in patient’s blood. The aim of the study was to evaluate the feasibility of deriving chromosome-edited E. coli clones producing T. gondii antigens. Here, we present the CRISPR-Cas9 facilitated editing of the E. coli genome to produce SAG2 and GRA2 T. gondii antigens. Moreover, we have derived a clone that produces both proteins and an additional clone producing a novel fusion protein, SAG2-GRA2. These proteins, bearing His-tag, can be easily purified and are useful to detect anti-Toxoplasma antibodies in human blood. We conclude that it is feasible to edit the E. coli chromosome to produce T. gondii antigens that are bound by antibodies present in people affected by toxoplasmosis. |
| Databáze: | OpenAIRE |
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