The Effects of Immunizing Sheep with Different BMP15 or GDF9 Peptide Sequences on Ovarian Follicular Activity and Ovulation Rate1
| Autor: | Peter Smith, Derek A. Heath, L G Moore, Karen L Reader, Kenneth P. McNatty, Lynda Whiting, Norma L. Hudson, Andrea Western, Stan Lun, Jennifer L. Juengel |
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| Rok vydání: | 2007 |
| Předmět: |
chemistry.chemical_classification
endocrine system medicine.medical_specialty biology Bone morphogenetic protein 15 media_common.quotation_subject chemical and pharmacologic phenomena Peptide Ovary Cell Biology General Medicine Growth differentiation factor-9 complex mixtures Endocrinology medicine.anatomical_structure Reproductive Medicine chemistry Internal medicine Follicular phase biology.protein medicine Antibody Ovulation Keyhole limpet hemocyanin media_common |
| Zdroj: | Biology of Reproduction. 76:552-560 |
| ISSN: | 1529-7268 0006-3363 |
| DOI: | 10.1095/biolreprod.106.054361 |
| Popis: | The aims of these studies were to determine the abilities of antisera against different regions of ovine bone morphogenetic protein 15 (BMP15) and growth differentiation factor 9 (GDF9) to inhibit ovarian follicular activity, estrus (mating), and ovulation in sheep. The 9–15-mer peptides were conjugated to keyhole limpet hemocyanin (KLH) and used to generate antibodies against the flexible N-terminal regions of the mature protein as well as against regions in which dimerization of the protein or interaction with a type 1 BMP or a type 2 TGFB or BMP receptor was predicted to occur. Ewes (n = 10 per treatment group) were vaccinated with KLH or the KLH-BMP15 (n = 9 different peptides) or KLH-GDF9 (n = 10) peptides in Freund adjuvant at five consecutive monthly intervals. Overall, antisera generated against peptides that corresponded to amino acid residues 1–15 of the N-terminus of the BMP15 or GDF9 mature protein or GDF9 amino acid residues 21–34 were the most potent at inhibiting ovulation followin... |
| Databáze: | OpenAIRE |
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