Differential Transcriptome Analysis of Cervus elaphus songaricus and Cervus elaphus yarkandensis Reveals Candidate Genes for Antler Regeneration
| Autor: | Zh. Ren, P. Zhao, Y. Jin, Sh. Wang, Ch. Han, Zh. Ma, D. Ni, W. Liu |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0303 health sciences Candidate gene animal structures Regeneration (biology) Zoology Biology 01 natural sciences Antler Transcriptome 03 medical and health sciences Bioinformatics software Genetics Cervus elaphus KEGG Gene 030304 developmental biology 010606 plant biology & botany |
| Zdroj: | Russian Journal of Genetics. 56:324-332 |
| ISSN: | 1608-3369 1022-7954 |
| DOI: | 10.1134/s1022795420030187 |
| Popis: | Antler is of high medicinal value as a traditional Chinese medicine and it has a high economic value. Both Cervus elaphus songaricus and Cervus elaphus yarkandensis are important species of Cervus elaphus yarkandensis in China, but they are different in the level of deer antlers production. So total RNA was extracted from frozen antler tissue and performed RNA-seq. Data from RNA-seq was disposed with multiple bioinformatics software. The expression of differential genes were evaluated by q-PCR. Based on the RNA-seq date of antlers, we obtained 385,712 unigenes by employing de novo assembly and then they were annotated in some databases, such as Nr, Swissprot, GO and KEGG. Combined with DGE technology, transcriptome differences between Cervus elaphus songaricus and Cervus elaphus yarkandensis were detected (>±2 Fold, P-value |
| Databáze: | OpenAIRE |
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