Transgenic Belarussian-bred potato plants expressing the genes for antimicrobial peptides of the cecropin-melittin type

Autor:	T. G. Tretyakova, T. A. Gapeeva, N. L. Vutto, I. D. Volotovski, A. N. Pundik
Rok vydání:	2010
Předmět:	biology fungi Antimicrobial peptides food and beverages Genetically modified crops Agrobacterium tumefaciens Plant disease resistance Erwinia biology.organism_classification Microbiology Transformation (genetics) Cecropin Genetics Cauliflower mosaic virus
Zdroj:	Russian Journal of Genetics. 46:1433-1439
ISSN:	1608-3369 1022-7954
DOI:	10.1134/s1022795410120057
Popis:	Binary vectors for Agrobacterium-mediated transformation were constructed to express the genes for antimicrobial peptides (APs) of the cectropin-melittin type under the control of the cauliflower mosaic virus 35S RNA promoter in plants. It was shown with Escherichia coli and Agrobacterium tumefaciens cells that the cassettes could be cloned in pBI121-based vectors with deletion of the β-D-glycuronidase gene only in the orientation opposite to that of the original vector. Transgenic potato plants were obtained using the Belarussian varieties Odyssey, Vetraz, and Scarb. Their cells expressed the MsrA1 or CEMA peptides of the cecropin-melittin type. The expression was shown to confer higher resistance to bacterial (Erwinia carotovora) infection and extremely high resistance to fungal (Phytophtora infestans and Alternarla solani) infections.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::86b5653706a1b363341aa650be1b823e https://doi.org/10.1134/s1022795410120057 Zobrazit plný text záznamu Full text from SpringerLink