Effects of Nω-nitro-L-arginine methyl ester and aminoguanidine on lipopolysaccharide-induced airway hyperresponsiveness in guinea pigs
| Autor: | Li Li, Xue-Hua Chen, Jie-Ming Qu, Li-xian He, Jue Pan, Yin-Xiang Cao, Da-Nian Zhu, Linlin Shen, Hong-ni Jiang |
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| Rok vydání: | 2008 |
| Předmět: |
medicine.diagnostic_test
Lipopolysaccharide Nω nitro l arginine methyl ester biology Airway hyperresponsiveness General Medicine respiratory system Pharmacology respiratory tract diseases Nitric oxide Nitric oxide synthase chemistry.chemical_compound Bronchoalveolar lavage chemistry Immunology medicine biology.protein Bronchoconstriction medicine.symptom Airway |
| Zdroj: | Chinese Medical Journal. 121:1693-1697 |
| ISSN: | 0366-6999 |
| DOI: | 10.1097/00029330-200809010-00020 |
| Popis: | BACKGROUND The down-regulation of constitutive nitric oxide synthase (cNOS) and up-regulation of inducible nitric oxide synthase (iNOS) are associated with the allergen-provocated airway hyperresponsiveness (AHR). This study aimed to determine whether their alteration also plays an important role in the AHR induced by lipopolysaccharide (LPS). METHODS Hartley male guinea pigs, weighing between 250 g and 350 g, were injected with LPS at a dose of 1 mg/kg every 24 hours for three days. A non-selective NOS inhibitor, N(omega)-nitro-L-arginine methyl ester (L-NAME), or a selective inducible NOS inhibitor, aminoguanidine (AG), were used thirty minutes before each injection of LPS. Airway reactions, nitric oxide (NO) production and inflammatory changes were detected 24 hours after the last dose of LPS. RESULTS AG significantly decreased the NO production in the bronchoalveolar lavage fluid (BALF) and sharply reduced the intensity of bronchoconstriction to histamine challenge. L-NAME also significantly decreased the NO production in the BALF, but had no effect on airway reactions or, perhaps, a tendency to enhance the intensity of AHR. CONCLUSIONS The data suggest that inducible NOS contributes to the AHR induced by repetitive intraperitoneal LPS, and constitutive NOS was also involved. |
| Databáze: | OpenAIRE |
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