Encapsulated Hsp70 decreases endotoxin-induced production of ROS and TNFα in human phagocytes
| Autor: | Antonova O.Yu., Michael B. Evgen'ev, L. I. Shabarchina, Kochetkova O.Yu., M. G. Vinokurov, M. M. Yurinskaya, A. V. Suslikov |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification Reactive oxygen species Innate immune system 030102 biochemistry & molecular biology Cell Biology Biology Biochemistry Molecular biology Polyelectrolyte Hsp70 Cell biology law.invention 03 medical and health sciences 030104 developmental biology chemistry law Heat shock protein Recombinant DNA Tumor necrosis factor alpha Secretion |
| Zdroj: | Cell Stress and Chaperones. 22:163-171 |
| ISSN: | 1466-1268 1355-8145 |
| DOI: | 10.1007/s12192-016-0743-z |
| Popis: | Human heat shock protein Hsp70 was experimentally inserted into polyelectrolyte microcapsules. Encapsulated recombinant Hsp70 was studied in terms of its effects on neutrophil apoptosis, the production of reactive oxygen species, and the secretion of tumor necrosis factor alpha by promonocytic THP-1 cells. It was found that encapsulated Hsp70 effectively inhibits neutrophil apoptosis, unlike free exogenous protein used in solution. In THP-1 cells, encapsulated and free Hsp70 reduced LPS-induced tumor necrosis factor alpha production with a similar efficiency. Encapsulated Hsp70 reduces LPS-induced reactive oxygen species production by neutrophils in the course of its release from the microcapsules but not as much as free Hsp70. Thus, the polyelectrolyte microcapsules can be used as containers for the effective delivery of Hsp70 to neutrophils and monocytes to significantly improve the functioning of the innate immune system. |
| Databáze: | OpenAIRE |
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