Chitosan polymer as support to IgG immobilization for piezoelectric applications
| Autor: | Rosa F. Dutra, Karla Patrícia de Oliveira Luna, Renata Maria Costa Souza, Rosângela Ferreira Frade de Araújo, Danyelly Bruneska, José Luiz de Lima Filho, Cosme Rafael Martínez |
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| Rok vydání: | 2013 |
| Předmět: |
chemistry.chemical_classification
Materials science Chromatography General Physics and Astronomy Surfaces and Interfaces General Chemistry Polymer Quartz crystal microbalance Condensed Matter Physics Piezoelectricity Surfaces Coatings and Films Chitosan Crystal chemistry.chemical_compound Adsorption chemistry Electrode |
| Zdroj: | Applied Surface Science. 274:33-38 |
| ISSN: | 0169-4332 |
| DOI: | 10.1016/j.apsusc.2013.02.046 |
| Popis: | Immunoenzymatic assays using gold plates and quartz crystal microbalance (QCM) analysis were carried out in order to evaluate chitosan/IgG interaction. Two chitosan solutions (S1 and S2) were prepared with different concentrations of NaOH (0.8% – S1 and 8% – S2). Absorbances 3-fold higher were obtained when chitosan (S2) was used as support when compared with direct IgG adsorption on gold. S1 on gold showed a better stability (at 22 °C, for 72 h) for IgG immobilization when compared with S2. However, S1 was used on QCM analysis and the IgG adsorption led to a non-Sauerbrey response, in which the mass on the electrode surface promote a proportional increase in the crystal resonant frequency. Direct IgG adsorption on gold electrode led to a 14.19% (±2.43) increase in crystal frequency. When S1 was used as a support for IgG, a better immobilization occurred, causing a 24.34% (±0.75) variation in crystal frequency. The structure of chitosan was shown to be efficient for IgG immobilization both in the immunoenzymatic method and in the QCM system. |
| Databáze: | OpenAIRE |
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