Blood transmission studies of prion infectivity in the squirrel monkey (S aimiri sciureus ): the Baxter study
| Autor: | Thomas R. Kreil, Diane Ritchie, Susan V. Gibson, Paul Brown, James W. Ironside, Christian R. Abee |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Blood transfusion medicine.medical_treatment Immunology Population Buffy coat Asymptomatic 03 medical and health sciences mental disorders medicine Immunology and Allergy education Whole blood Infectivity education.field_of_study biology Squirrel monkey Saimiri sciureus Hematology biology.organism_classification Virology nervous system diseases 030104 developmental biology medicine.symptom |
| Zdroj: | Transfusion. 56:712-721 |
| ISSN: | 0041-1132 |
| DOI: | 10.1111/trf.13422 |
| Popis: | BACKGROUND Four secondary transmissions of variant Creutzfeldt-Jakob disease (vCJD) infectivity have been associated with the transfusion of nonleukoreduced red blood cells collected from vCJD patients during the asymptomatic phase of the disease. Establishing efficient experimental models for assessing the risk of future transmissions of vCJD infectivity via blood transfusion is of paramount importance in view of a study of archived appendix samples in which the prevalence of asymptomatic vCJD infection in the United Kingdom was estimated at approximately 1 in 2000 of the population. In this study, we investigated transmission of vCJD and sporadic CJD (sCJD) infectivity from blood using the squirrel monkey, which is highly susceptible to experimental challenge with human prion disease. STUDY DESIGN AND METHODS Whole blood collected from vCJD- and sCJD-infected squirrel monkeys was transfused at multiple time points into recipient squirrel monkeys. Blood recipients were euthanized approximately 7 years after their first blood transfusion. RESULTS No clinical or pathologic signs of a prion disease were observed in either the sCJD- or the vCJD-transfused monkeys, and immunohistochemistry and biochemical investigations showed no PrPTSE in central nervous system or lymphoreticular tissues. Similarly, monkeys inoculated intracerebrally (IC) and intravenously (IV) with either buffy coat or plasma from vCJD and sCJD patients failed to develop disease. However, white blood cells from a chimpanzee-passaged strain of human Gerstmann-Straussler-Scheinker (GSS) disease transmitted autopsy-proven disease to two IC-inoculated monkeys after incubation periods of 34 and 39 months. CONCLUSION Blood transmits GSS but not sCJD or vCJD infectivity to IC- or IV-inoculated squirrel monkeys within a 7-year observation period. |
| Databáze: | OpenAIRE |
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