Hepatic fat and adenosine triphosphate measurement in overweight and obese adults using 1H and 31P magnetic resonance spectroscopy
| Autor: | Stephen O. Crawford, Jeanne M. Clark, Anna Mae Diehl, Susanne Hemker, Charalett E. Diggs, Frederick L. Brancati, Steven F. Solga, Alena Horská |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Hepatitis
medicine.medical_specialty Cirrhosis Hepatology medicine.diagnostic_test business.industry Fatty liver Magnetic resonance imaging Overweight medicine.disease Gastroenterology law.invention Endocrinology Randomized controlled trial law Diabetes mellitus Internal medicine Cohort Medicine medicine.symptom business |
| Zdroj: | Liver International. 28:675-681 |
| ISSN: | 1478-3223 |
| DOI: | 10.1111/j.1478-3231.2008.01705.x |
| Popis: | Hepatic magnetic resonance spectroscopy (MRS) is increasingly being applied to many areas of patient-oriented liver research (1). Proton MRS (1H MRS) can quantify triglyceride content in the liver (2) and has become well-established for this purpose (3). Phosphorus MRS (31P MRS) can evaluate complex metabolic processes in vivo and has been applied to many areas of clinical liver research, including investigations of cirrhosis, hepatitis, obstructive jaundice and hepatic energy homoeostasis (4–7). MRS uses standard magnetic resonance equipment, is noninvasive and imparts little risk on study subjects. Further, the data generated by 31P MRS are unique. For example, no other research modality can assess hepatic energy in vivo as indicated by hepatic adenosine triphosphate (ATP) levels. Despite these important attributes, some obstacles are faced by investigators interested in human hepatic MRS. Human hepatic MRS protocols, in particular 31P MRS protocols, can be technically challenging, time consuming and costly. There is considerable variation in data analysis techniques in the published literature, and all share the potential for systematic and random error (8). Perhaps most important, the reported intra- exam and intrasubject variability ranges widely in published studies (9, 10). Furthermore, the effect of fasting or time of day on hepatic ATP levels is uncertain. Accordingly, it is important to understand the technical factors associated with cost-efficient and successful MRS data acquisition and analysis. In this paper, we report our experience with both hepatic 1H and 31P MRS in a cohort of overweight and obese diabetic subjects enrolled in the Action for Health in Diabetes (Look AHEAD) study compared with healthy controls. We also report inter- and intra-observer variability in the evaluation of spectra, and the stability of MRS measurements through repeated examinations on healthy volunteers. We hypothesized that we could successfully complete 1H and 31P MRS in a reliable fashion in obese adults. |
| Databáze: | OpenAIRE |
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