LD-RTPCR: A new method for labelling trace cDNA microarray probe

Autor: Hao Liang, Jing-fen Sun, De-chang Wu, Sheng-qi Wang, Ping-Kun Zhou, Bao-xing Fan
Rok vydání: 2002
Předmět:
Zdroj: Chinese Journal of Cancer Research. 14:179-182
ISSN: 1993-0631
1000-9604
DOI: 10.1007/s11670-002-0040-5
Popis: Objective: To explore the usefulness of long distance reverse transcript combining linear amplification (LD-RTPCR) in labeling slight trace probe used for cDNA microarray. Methods: Total RNA from BEP2D cells was extracted and labeled by two different methods, LD-RTPCR with Cy3-dCTP as fluorescent dye and traditionally used RNA reverse transcript (RT) with Cy5-dCTP as fluorescent dye. Then, the probes labeled by two methods were mixed equally and hybridized with the cDNA microarray. Results: Scan and analysis of the microarray showed that the two methods labeled probes had consistent results. Conclusion: LD-RTPCR was proved useful for labeling cDNA microarray probe, especially for limited RNA material.
Databáze: OpenAIRE
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