T lymphocyte expression of thrombospondin-1 and adhesion to extracellular matrix components

Autor: Sten-Erik Bergström, Ignacio Arencibia, Anders Sandström, Shu Shun Li, Anna Ivanoff, Birger Christensson, Dan Hauzenberger, Joost van Nerven, Karl-Gösta Sundqvist, Jan Holgersson
Rok vydání: 2002
Předmět:
Zdroj: European Journal of Immunology. 32:1069-1079
ISSN: 1521-4141
0014-2980
DOI: 10.1002/1521-4141(200204)32:4<1069::aid-immu1069>3.0.co;2-e
Popis: The mechanisms controlling the formation of pseudopodia and other active cell edges in T lymphocytes are not understood. We show here that T lymphocytes express thrombospondin-1 (TSP-1). TSP-1 in T lymphocytes has a high turnover as shown by the fact that brefeldin and monensin rapidly increase while cycloheximide tend to decrease the cellular TSP-1 content. T cell TSP-1 is preferentially stored intracellularly and shows variable cell surface expression. T lymphocyte adhesion to fibronectin and collagen type IV induces TSP-1 expression on the cell surface via a brefeldin sensitive mechanism. A monoclonal antibody to TSP-1 inhibits the flattening and pseudopodia formation of the adherent T cells. Furthermore, the same antibody to TSP-1 also exerts an inhibitory effect on T cell migration in the absence of exogenous TSP-1. These results indicate that endogenous TSP-1 is part of an adhesion-dependent mechanism controlling cytoplasmic spreading and migration in T lymphocytes.
Databáze: OpenAIRE
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