Ca2+channels, ryanodine receptors and Ca2+-activated K+channels: a functional unit for regulating arterial tone

Autor:	Thomas Kleppisch, Adrian D. Bonev, Thomas J. Heppner, Andra S. Stevenson, Michael Rubart, Harm J. Knot, Mark T. Nelson, Valerie A. Porter, George C. Wellman, W. J. Lederer, Jonathan H. Jaggar, Maik Gollasch, Guillermo J. Pérez
Rok vydání:	1998
Předmět:	Membrane potential medicine.medical_specialty Vascular smooth muscle Physiology Ryanodine receptor Chemistry Depolarization Cardiac action potential Membrane hyperpolarization Hyperpolarization (biology) musculoskeletal system Endocrinology Internal medicine cardiovascular system medicine Biophysics Repolarization
Zdroj:	Acta Physiologica Scandinavica. 164:577-587
ISSN:	0001-6772
Popis:	Local calcium transients ('Ca2+ sparks') are thought to be elementary Ca2+ signals in heart, skeletal and smooth muscle cells. Ca2+ sparks result from the opening of a single, or the coordinated opening of many, tightly clustered ryanodine receptor (RyR) channels in the sarcoplasmic reticulum (SR). In arterial smooth muscle, Ca2+ sparks appear to be involved in opposing the tonic contraction of the blood vessel. Intravascular pressure causes a graded membrane potential depolarization to approximately -40 mV, an elevation of arterial wall [Ca2+]i and contraction ('myogenic tone') of arteries. Ca2+ sparks activate calcium-sensitive K+ (KCa) channels in the sarcolemmal membrane to cause membrane hyperpolarization, which opposes the pressure induced depolarization. Thus, inhibition of Ca2+ sparks by ryanodine, or of KCa channels by iberiotoxin, leads to membrane depolarization, activation of L-type voltage-gated Ca2+ channels, and vasoconstriction. Conversely, activation of Ca2+ sparks can lead to vasodilation through activation of KCa channels. Our recent work is aimed at studying the properties and roles of Ca2+ sparks in the regulation of arterial smooth muscle function. The modulation of Ca2+ spark frequency and amplitude by membrane potential, cyclic nucleotides and protein kinase C will be explored. The role of local Ca2+ entry through voltage-dependent Ca2+ channels in the regulation of Ca2+ spark properties will also be examined. Finally, using functional evidence from cardiac myocytes, and histological evidence from smooth muscle, we shall explore whether Ca2+ channels, RyR channels, and KCa channels function as a coupled unit, through Ca2+ and voltage, to regulate arterial smooth muscle membrane potential and vascular tone.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::868c8a071ed11ff6549e7ef4a9d422a0 https://doi.org/10.1046/j.1365-201x.1998.00462.x Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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