Pollen wall development inHypecoum imberbeSm. (Fumariaceae)

Autor:	Ana T. Romero, M. Carmen Fernández, Maria Salinas
Rok vydání:	2003
Předmět:	Foot layer Cell wall Tapetum Transmission electron microscopy Botany Ultrastructure Biophysics Plant Science Biology Ecology Evolution Behavior and Systematics Pollen wall Aperture (botany) Hypecoum imberbe
Zdroj:	Grana. 42:91-101
ISSN:	1651-2049 0017-3134
DOI:	10.1080/00173130310012521
Popis:	We have studied the ontogeny of the pollen wall of Hypecoum imberbe. Our initial observations with conventional transmission electron microscopy (TEM) were inconclusive due to the similarities found in the electrodensity of the foot layer and the endexine, and between this latter layer and the intine. Thus we applied the PTA acetone histochemical test in order to differentiate between the cell wall components. This method proved to be efficient in resolving the differences between the layers and even allowed us to distinguish two strata within the narrow intine layer. A thin foot layer can be distinguished only by the temporary presence of a single white line separating it from the initial deposition of the endexine. The aperture consists of two colpi with no special differentiation. The tapetum is a typical secretory type giving rise to elaioplasts and tapetosomes during development, which persist as individual organelles upon the degradation of the tapetum. As the ultrastructural organisation of the spo...
Databáze:	OpenAIRE
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