Refractometric detector for a microcolumn high-performance size-exclusion and micro gel chromatograph

Autor:	B.G. Belen'kii, Maxim L. Alexandrov, Jeno E. Kever, Gotlib Vladimir A
Rok vydání:	1992
Předmět:	chemistry.chemical_classification Detection limit Chromatography Chemistry Capillary action Mechanical Engineering Detector Size-exclusion chromatography Analytical chemistry Filtration and Separation Polymer Gel permeation chromatography Signal-to-noise ratio (imaging) Root-mean-square deviation
Zdroj:	Journal of Microcolumn Separations. 4:385-392
ISSN:	1520-667X 1040-7685
DOI:	10.1002/mcs.1220040504
Popis:	It is shown that an interference-polarizing refractometric detector makes it possible to use microcolumn size-exclusion chromatography (MSEC) columns with inner diameters of 0.2 to 0.5 mm and ensures a detection limit (at a signal to noise ratio of 3) of 8 × 10−8 refractive index units. Taking into account the above requirements, a micro-gel chromatograph with a refractometric (interference-polarizing) detector was developed. Teflon columns gave reproducibilities (the mean square deviation at the 95% confidence level) of retention times and signal amplitudes of 0.2% and 0.5%, respectively, which ensures reproducibilities of molecular weight determinations within about 2 to 4%. Examples of polymer and protein analyses carried out on this chromatograph are given. It is suggested that a micro-gel chromatograph can be developed for fused-silica capillary columns with inner diameters of 0.2 mm. This would permit analysis of approximately 10 pg of polymer.
Databáze:	OpenAIRE
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