Development of DNA probes for the identification of sibling species A of the Anopheles culicifacies (Diptera: Culicidae) complex

Autor:	H.G. Nandadasa, E.H. Karunanayake, Wimaladharma Abeyewickreme, Maya B. Gunasekera, S.K. Subbarao, B.G.D.N.K. De Silva
Rok vydání:	1995
Předmět:	Genetics Species complex Hybridization probe Zoology General Medicine Biology biology.organism_classification DNA sequencing chemistry.chemical_compound Sensu chemistry Insect Science Molecular probe Anopheles culicifacies Repeated sequence Agronomy and Crop Science DNA
Zdroj:	Bulletin of Entomological Research. 85:345-353
ISSN:	1475-2670 0007-4853
DOI:	10.1017/s0007485300036075
Popis:	Three highly repetitive DNA sequences Rp36, Rp217 and Rp234, have been isolated from Anopheles culicifacies Giles sensu lato. The cloned DNA sequences were found at a higher copy number in species B and C, than in species A of the A. culicifacies complex. These sequences may therefore be used as DNA probes to distinguish species A from the other two species, using a 200-fold dilution of a single mosquito DNA extract in a dot-blot hybridization assay. Rp36 and Rp217 have been completely sequenced. Internal repeats were absent in Rp36. Two related core sequences of 13 and 16 bp were found tandemly repeated in Rp217. These probes enable the rapid detection of species A of A. culicifacies in field investigations.
Databáze:	OpenAIRE
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